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Metformin restores insulin sensitivity but not glucose tolerance during Mtb infection. Duncan-Hartley guinea pigs received oral administration of 25 mg/kg metformin or mock treatment once daily beginning 28 days prior to low-dose aerosol infection with Mtb H37Rv. Thirty days following infection guinea pigs were subjected to an insulin tolerance test by injecting 0.5 units/kg human <t>recombinant</t> insulin SC ( A ) or an oral glucose tolerance test by challenging fasted animals with a 2 g/kg bolus of d -glucose ( B ). Blood glucose concentrations were measured at 0, 25, 50, 75, and 100 min post insulin injection and 0, 30, 60, 90, and 120 min post oral glucose challenge. A 2-factor mix model was used to determine significances with Tukey’s correction for multiple comparisons. n = 9, *** p ≤ 0.001 ; ** p ≤ 0.01.
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Metformin restores insulin sensitivity but not glucose tolerance during Mtb infection. Duncan-Hartley guinea pigs received oral administration of 25 mg/kg metformin or mock treatment once daily beginning 28 days prior to low-dose aerosol infection with Mtb H37Rv. Thirty days following infection guinea pigs were subjected to an insulin tolerance test by injecting 0.5 units/kg human <t>recombinant</t> insulin SC ( A ) or an oral glucose tolerance test by challenging fasted animals with a 2 g/kg bolus of d -glucose ( B ). Blood glucose concentrations were measured at 0, 25, 50, 75, and 100 min post insulin injection and 0, 30, 60, 90, and 120 min post oral glucose challenge. A 2-factor mix model was used to determine significances with Tukey’s correction for multiple comparisons. n = 9, *** p ≤ 0.001 ; ** p ≤ 0.01.
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Metformin restores insulin sensitivity but not glucose tolerance during Mtb infection. Duncan-Hartley guinea pigs received oral administration of 25 mg/kg metformin or mock treatment once daily beginning 28 days prior to low-dose aerosol infection with Mtb H37Rv. Thirty days following infection guinea pigs were subjected to an insulin tolerance test by injecting 0.5 units/kg human <t>recombinant</t> insulin SC ( A ) or an oral glucose tolerance test by challenging fasted animals with a 2 g/kg bolus of d -glucose ( B ). Blood glucose concentrations were measured at 0, 25, 50, 75, and 100 min post insulin injection and 0, 30, 60, 90, and 120 min post oral glucose challenge. A 2-factor mix model was used to determine significances with Tukey’s correction for multiple comparisons. n = 9, *** p ≤ 0.001 ; ** p ≤ 0.01.
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recombinant human insulin humulin regular  (Eli Lilly)
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Metformin restores insulin sensitivity but not glucose tolerance during Mtb infection. Duncan-Hartley guinea pigs received oral administration of 25 mg/kg metformin or mock treatment once daily beginning 28 days prior to low-dose aerosol infection with Mtb H37Rv. Thirty days following infection guinea pigs were subjected to an insulin tolerance test by injecting 0.5 units/kg human <t>recombinant</t> insulin SC ( A ) or an oral glucose tolerance test by challenging fasted animals with a 2 g/kg bolus of d -glucose ( B ). Blood glucose concentrations were measured at 0, 25, 50, 75, and 100 min post insulin injection and 0, 30, 60, 90, and 120 min post oral glucose challenge. A 2-factor mix model was used to determine significances with Tukey’s correction for multiple comparisons. n = 9, *** p ≤ 0.001 ; ** p ≤ 0.01.
Recombinant Human Insulin Humulin Regular, supplied by Eli Lilly, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+human+regular+insulin/recombinant+human+insulin+humulin+r/pm29751248-95-9-13
Average 90 stars, based on 1 article reviews
recombinant human insulin humulin regular - by Bioz Stars, 2026-10
90/100 stars
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Metformin restores insulin sensitivity but not glucose tolerance during Mtb infection. Duncan-Hartley guinea pigs received oral administration of 25 mg/kg metformin or mock treatment once daily beginning 28 days prior to low-dose aerosol infection with Mtb H37Rv. Thirty days following infection guinea pigs were subjected to an insulin tolerance test by injecting 0.5 units/kg human recombinant insulin SC ( A ) or an oral glucose tolerance test by challenging fasted animals with a 2 g/kg bolus of d -glucose ( B ). Blood glucose concentrations were measured at 0, 25, 50, 75, and 100 min post insulin injection and 0, 30, 60, 90, and 120 min post oral glucose challenge. A 2-factor mix model was used to determine significances with Tukey’s correction for multiple comparisons. n = 9, *** p ≤ 0.001 ; ** p ≤ 0.01.

Journal: Scientific Reports

Article Title: Metformin enhances protection in guinea pigs chronically infected with Mycobacterium tuberculosis

doi: 10.1038/s41598-020-73212-y

Figure Lengend Snippet: Metformin restores insulin sensitivity but not glucose tolerance during Mtb infection. Duncan-Hartley guinea pigs received oral administration of 25 mg/kg metformin or mock treatment once daily beginning 28 days prior to low-dose aerosol infection with Mtb H37Rv. Thirty days following infection guinea pigs were subjected to an insulin tolerance test by injecting 0.5 units/kg human recombinant insulin SC ( A ) or an oral glucose tolerance test by challenging fasted animals with a 2 g/kg bolus of d -glucose ( B ). Blood glucose concentrations were measured at 0, 25, 50, 75, and 100 min post insulin injection and 0, 30, 60, 90, and 120 min post oral glucose challenge. A 2-factor mix model was used to determine significances with Tukey’s correction for multiple comparisons. n = 9, *** p ≤ 0.001 ; ** p ≤ 0.01.

Article Snippet: Similarly, insulin sensitivity was measured following the SC injection of 0.5 units/kg of regular-acting human recombinant insulin (Humulin-R, Eli Lilly).

Techniques: Infection, Aerosol, Recombinant, Injection